Sydney born, Luise Napier began singing at the age of 9 competing in the Sydney, Railway and St. George Eisteddfods. Her first amateur performances began when she was 16 with her first professional engagements for JC Williamson's from 1966–1970. She joined The Australian Opera Chorus in 1973 for the opening of the Sydney Opera House and spent eleven years as a chorister, singing in approximately 48 operas. As a principal singer, performing over 30 various roles and understudies Luise also sang for Opera Queensland and in the Adelaide Festival. As Resident Director with OA Luise spent over 21 years assisting and directing over fifty-five operas including her own restudied version of Rinaldo in 2005. She revived many OA productions in Sydney, Melbourne, Adelaide, Perth, Brisbane, New Zealand and directed Opera in the Domain and Opera in the Bowl in Melbourne in 2004 as well as Tosca and Romeo & Juliet for Opera Queensland.
Luise resigned from OA in 2009 with her first job as a freelance director, in the UK, as Associate Director of Opera North's production of Rusalka in 2010. In 2011 she assisted the Artistic Director of Pacific Opera, briefly taking over the position, working with the Young Artists and directing the major fundraising Gala Event in 2011. She was a member of Opera Foundation Australia's Music Board from 2007-2010, which included adjudicating and conducting Workshops for various Awards and Scholarships.